𝚃𝚛𝚢𝚒𝚗𝚐 𝚝𝚘 𝚜𝚝𝚘𝚙 𝚜𝚊𝚍 𝚝𝚑𝚘𝚞𝚐𝚑𝚝𝚜

Heh.

It's been a while, hasn't it?

(ദ്ദി˙ᗜ˙)

I don't know if anyone actually checks these posts. I assume not, judging by the views they get. On one hand, I'm glad that's the case because I don't know if I'll really be ready for the criticism and potential memes or jokes, which are bound to happen. I even participate in the jokes myself on Instagram.

Haha...

૮₍´˶• . • ⑅ ₎ა

૮₍´˶• . • ⑅ ₎ა

૮₍´˶• . • ⑅ ₎ა

(ㅇㅅㅇ)

⊂(◉‿◉)つ

(♥‿♥)

╭(╭⊚‿⊚╮)╮

:00000  (˶˃ ᵕ ˂˶)

so fucking yay

⸜(｡˃ ᵕ ˂ )⸝♡

(⸝⸝๑  ̫ ๑⸝⸝⸝)

(⸝⸝⸝-﹏-⸝⸝⸝)

✧(⸝⸝⸝ᵒ̴̶̷ ｡ ᵒ̴̶̷⸝⸝⸝)

✩₊˚.⋆☾⋆⁺₊✧

This anime has definitely become one of my favorites. In fact, before I continue writing, I'm going to post my review on MyAnimeList. Well, I'm back. I'll leave some images here, but I have to say I really liked the plot, much more than I expected. The divorce subplot might be a heavy or even annoying thing to consider, but for me it's important because... it's precisely about how to cope with family problems. Arguments. My mother is yelling at me right now, so it's difficult for me to write this. That's why I consider it important. The ending was also very beautiful. The episode's ending. It's a small escape from reality, well, at least from my reality. Mother's screams sound more and more like noise or vibrations in my head; it's a strange feeling. They sound threatening and make me want to run to my room and cry for a while. Then I remember I don't have a room and that I just have to keep going and pretend everything is okay. And in the end, I think that's what my life is all about, as it always has been. When something was stolen from me at school, or something was broken, well, that's when I cried because I knew I was in for a beating from my mother. It continued in later stages, with me staying quiet and putting up with it. Putting it up with it until I suppose I couldn't anymore.

The end of the episode was simply beautiful, how they finally establish a relationship, accept the problems that might eventually arise, and underlyingly accept that they will try to solve them together. Why has humankind evolved to such an extent that it's so necessary to feel accompanied in life? I could break down that answer well as a student of Evolutionary Biology. But I don't feel like doing it right now, and maybe never will.

It's a good time to say that I finally took my computer home (to my mother's; I don't have my own place, obviously). It was with some technicians for a couple of days, and they left it just as damaged. The problem now seems to be the video card, well, the video connections to the screen, or whatever that's called. I don't even deserve a new device. What I do is wait for it to turn on so I can work on parts of my Final Project as best I can, and when it doesn't, I play Brawl Stars or make some progress on my illustrations.

I don't deserve a new computer; I've always been useless. Even now, I'm wasting time watching my favorite anime and playing Roblox. And writing this post. It's fine! It can't be fixed right away. Tomorrow, I'm supposed to go to my internship at the Museum, but I don't even feel like it. My mom keeps saying, "It's time to start saving for the bus fare," which isn't cheap since I live far away. I have to emphasize that, why? To justify my lack of motivation, and also because, even though it bothers me, it's true... and generally, I think I'm going to drop out. Clearing my head somewhere else is expensive. And uncomfortable; the buses are cramped, and having to deal with other people staring is annoying too. My pants don't fit me as well as I'd like.

I don't like staying home and being constantly under my mother's thumb, enjoying the good times with her when I know that if something goes wrong, the atmosphere completely changes and the negativity returns. In fact, it just happened again, something with my damn braces (and let me tell you, if I'm being completely honest, I would have preferred to invest that money I've already spent on therapy and a makeover, rather than on teeth that I don't even think make me smile properly, although they do seem straighter).

The negativity I feel right now is enough to make me want to go to the orthodontist and tell him, "I won't have time because of my studies. I'm sorry."

But I'll try to hold out a little longer until I finish writing everything here.

Anyway, what I was getting at is: I don't really like the museum because it's obvious those jars with large animals contain a lot of formaldehyde. The curator says it's just alcohol and that it's fine, so much so that he actually believes it himself and handles the specimens with his bare hands. I also think he thinks I was born yesterday; that stuff obviously has formaldehyde or something in it. The smell is distinctly that of regular ethanol, the atmosphere is heavy for someone who isn't always there, and it irritates me. These reasons are also a burden for me to want to leave, and... why did I go in the first place?

Well, one reason was to find a good excuse to escape the house for a while. But it's certainly not giving me much peace, because the issue of the cost of lunches and such came up. I've actually been thinking that I could just keep going, but cut back on the lunches and save that money for some future treat, like an escort. Because, to be honest, I don't think I'll ever be able to find someone special or someone who's a good match for me. "If I improve, yes," but I still see that as a long way off (unattainable).

Another reason I went into that museum was to see if I could find that special someone, but it's not going to happen. It's a pretty lame goal, so the result is going to be lame too. A student intern from a nearby university recently started there, so I immediately began a conversation with her until the topic of relationships came up, and she has a boyfriend. So, that's it. I simply limited myself to greetings or talking if she initiated it, during the internship. I'm not going to make the same mistake as with () of ultimately wanting to simulate what it would be like to have a girlfriend who fits in, or worse, of groveling for attention like I did with girl M. Looking back, that was truly pathetic; it must have been bad even for her. I was just looking for a partner, and that was clearly a forced interaction where she only saw me as a casual companion.

So what I'm trying to do is keep shifting my focus away from people, especially couples, and try to reach at least my goal of 100 protist illustrations, as much as I can. Should I keep going to the museum? I don't know. If I want to keep getting those free lunches, I could go to the library and spend my time there, saving up for lunches. Although I don't know if I'll be able to make it through a whole day until the afternoon, pretending I'm actually going to the museum. That's partly why I don't want to leave the museum, and also because "I could get a volunteer certificate that would look good on my poor old CV."

Hmmm... maybe I'll stick around at that museum this week, so my departure isn't so abrupt. I definitely won't go tomorrow, because I promised an acquaintance I'd help him identify some aquatic insects.

I'll see how I can structure my time and my demons.

And long live the melancholic music of the last century.

( ´ ▽ ` )b

Pygsuia biforma Brown et al. 2013

Another one of the strange organisms I've been illustrating. I find the name funny because no matter how I look at it, it sounds like "pig." The name "biforma" is because it has two forms, indeed (obviously lol). Unlike Subulatomonas, which I was too lazy to illustrate, I actually illustrated both forms of P. biforma. I had it all ready when I realized a structure was missing and I had to redo it—what a pain!

Reminder (da f___ng reminder) that the images of the organism are free to use under CC BY-SA 4.0, non-commercial, attribution required (DOTkamina 2026).

If you've ever stumbled across this obscure site, you'll know I'm a ₗₐzy bᵤₘ, so I'll take advantage of the fact that this organism is also a breviate (like Subulatomonas tetraspora), and copy the exact same taxonomic description, varying it for Pygsuia biforma. I hope you can forgive me. Or well, never mind, I already did it before with cryptomonad algae. So, idc.

Pygsuia biforma is not in AlgaeBase, which surprised me. It is in NCBI Taxonomy. The organism belongs to the family Pygsuidae, order Breviatida, class Breviatea. The truth is, I've only found the name "Pygsuidae" on Wikipedia. NCBI Taxonomy directly includes P. biforma as part of Breviatea.

The class Breviatea, the breviate amoebas, are strange amoebas that lack mitochondria (instead, they have structures similar to them, as you'll see later), have two flagella, and a metabolic style adapted to low oxygen (anaerobic). They are unusual because their taxonomic placement is uncertain.

The class Breviatea is included in the clade Obazoa, a group of eukaryotes that also includes Apusomonadida (amoebas that do have mitochondria, although some have modifications that resemble those of Breviatea) (Torruella et al. 2018) and Opisthokonta (amoeboid eukaryotes that share the characteristic of moving with the aid of a single posterior flagellum. In contrast, Breviatea and Apusomonadida move with at least one anterior flagellum. Opisthokonta is notable for encompassing organisms related to the ancestors of animals and fungi, as well as the animals and fungi themselves).

Obazoa is grouped with Amoebozoa (the "common amoebas" as such) in the clade Amorphea or Unikonta (common characteristic: a single flagellum) (Spiegel 2016). Amorphea is included in the clade Podiata (which would include Amorphea and CRuMs). Podiata is finally included in the large domain Eukaryota, related to other clades I've already covered and others I hope to discuss later, such as Metamonada (Giardia lamblia) or Diaphoretickes (which includes Archaeplastida (plants and relatives of plant ancestors), Pancryptista (which includes cryptomonad algae), the SAR group, and so on)...

I have relied mainly on two articles for the creation of the illustrations of this organism, as well as for writing its description:

• "Phylogenomics demonstrates that breviate flagellates are related to opisthokonts and apusomonads". Matthew William Brown, Susan Sharpe, Jeffrey D. Silberman, Aaron A. Heiss, B. Franz Lang, Alastair Simpson, Andrew J. Roger. Proceedings of The Royal Society B. 280(1769):20131755. DOI: 10.1098/rspb.2013.1755. 2013.
• "A SUF Fe-S Cluster Biogenesis System in the Mitochondrion-Related Organelles of the Anaerobic Protist Pygsuia". Courtney W. Stairs, Laura Eme, Matthew W. Brown, Cornelis Mutsaers, Edward Susko, Graham Dellaire, Darren M. Soanes, Mark van der Giezen, Andrew J. Roger. Current Biology. Vol. 4, Issue 11. 1176-1186 pp. 2014.

Well, this organism is certainly interesting. Uhm... I'm sure it won't seem so interesting to me once I explore its taxonomic relatives further. I've represented its two main forms: adherent and swimming.

In both forms, we can see that the organism has a nucleus (I assume it also has a nucleolus, but Brown et al. 2013 don't mention this organelle, so I've decided not to represent it); an elongated, double-membrane mitochondrial-related organelle (MRO) (usually only one) without obvious cristae, one end of which is close to the basal bodies; bacteria ingested in food vacuoles (I've represented two "morphs"—the more oval vacuoles are inspired by Brown et al. 2013, and the comma-shaped ones are based on the micrographs by Stairs et al. 2014); and starch-like granules (bodies), which Brown et al. (2013) mentions that there are "several," although only one is indicated and observed in the micrograph Figure 1 of that article... but I have depicted more. 

Of course, we mustn't forget the basal bodies (the structures that attach to the flagellar microtubules), which are at an obtuse angle. There is also a Golgi apparatus near the anterior end of the MRO. I have depicted it almost elongated, as can be seen in Brown et al. (2013): Figure 1e. Oh, and I almost forgot: there's also a "dorsal microtubular fan" (MDF), which surrounds the back of the anterior basal body and appears to continue close to the MRO (see Brown et al. 2013: Figure 1e). I could have sworn the MDF was visible in the image, but looking at it again, I've drawn it very thin... which is close to reality, but it's still not very noticeable at first glance. I apologize for any potential eye strain.

I almost forgot this too: the endoplasmic reticulum is speculatively shaped, and I assume it exists because it's a nearly ubiquitous structure in all eukaryotic cells. The rough endoplasmic reticulum has ribosomes (those light-colored, stuck-together dots in the image), but I assume ribosomes are also dispersed throughout the rest of the cell. The smooth endoplasmic reticulum doesn't have nearly as many ribosomes. In this illustration, as well as in all the previous ones of other organisms, I've always depicted the smooth endoplasmic reticulum as having shorter and somewhat wider bodies than the rough endoplasmic reticulum, but that's just my convention to try and make the difference more noticeable. In real life, both forms can be intertwined, have the same width, and not be noticeable as two completely separate structures. I think I should have included that caveat for the others as well.

That said, let's get to the morphs. The cell in its adherent form is pear-shaped. Dimensions: 8.5–18.5 mm long and 5–8 mm wide. It has two flagella. The anterior or apical flagellum is the longer one, 8 to 30 µm. The posterior flagellum is (usually) shorter, measuring less than the cell itself. It's inserted subapically and closely associated with the cell surface, hence why I've drawn it attached to the cell, in the region where the MRO would be underneath. Anatomically It's very likely that the spatial arrangement I've chosen for the illustration won't always be the case. 

When the cell is actively gliding in an adherent state, it develops prominent, filose pseudopodia, which form at the anterior end of the cell. For a moment, I was freaking out because in Brown et al. 2013: Figure 1a, more pseudopodia are shown not only at the anterior end but also along one side of the cell, extending to the posterior end. But apparently, pseudopodia can form at the posterior end and on the rest of the cell (Brown et al. 2013: Supplementary Data Figure S6 B). The only difference is that the posterior pseudopodia are more prominent and appear to be branched. I haven't represented that property because I just found out about it. And honestly, I don't think I'm going to make a new image representing that possible state. Sorry!

Finally, the other state is the swimming cell form. Here, the cell has a more rounded or elongated shape (not obviously pear-shaped; I depicted it as a somewhat narrow oval, but not quite pear-shaped). Cells in this form are 8.5–13 mm long and 3.7–5.3 mm wide. The anterior or apical flagellum is "long", 8.5–28 mm long, and inserted apically. The main difference lies in the posterior flagellum. In the adherent form, this was short and attached to the cell surface, but in the swimming form, the posterior flagellum is 50% longer than the anterior flagellum (16–37 mm long), and, although still directed posteriorly, it is more "free," meaning it is not as tightly attached to the cell surface.

That's really all there is to say about this organism. 

ヾ(^ ^ゞ

I hope this information and the images are helpful. Don't forget to give credit, and read the original articles where I got all this information if you want more technical details and so on. 

I'm signing off now 'cause I want to upload this to Wikimedia Commons quickly.

(∩♡°ω°)⊃